Adventitious presence of GMO
Adventitious presence means the unintended, technically unavoidable presence of genetically engineered (GM) material in a product.
Adventitious presence is an unavoidable reality in plant biology, seed production and the distribution of crops. There are a number of factors that contribute to adventitious presence: Pollen flow, mixing during harvesting, transport, storage, processing, human error and accidents.
We offer an almost complete portfolio to determine the presence of AP material in rapeseed, maize, sugarbeet, soybean, cotton, rice and other crops.
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Rapeseed/canola: For complete GMO screening of rapeseed, a 5-target assay is recommended. The DNA targets screened for are the gox gene, the bar and pat genes, and the nptII and t-nos genes. An alternative is to screen only for commercial events, which are covered by the three targets gox, pat, and bar genes.
Maize: For maize a screen for p35s and t-nos is recommended. In the event of a positive sample, an analysis for identification of the GM event can be performed.
Rice: p35S, bar, t-nos, (nptII).
Sugarbeet: p35S alternative H7-1
Others: For wheat, sunflower and alfalfa, a screen for p35S and t-nos is recommended.
The test results are reported individually on accredited certificates, as well as together in an Excel overview. In the event of a positive sample, an estimate of the amount of GMO present in the sample can be provided.
Analyses of fresh leaves
Pooled leaves: Up to 100 pooled leaves (pieces of 6-8 mm in diameter) are homogenised and the DNA is extracted. Two sub-samples are tested by Real-Time PCR analyses for the presence of 2-5 GM traits and a species-specific reference gene.
Single leaves: Each leaflet (6-8 mm in diameter) is separately extracted for DNA and tested by Real-Time PCR analyses for the presence of 2-5 GM traits and a species-specific reference gene.
Upon request, remaining DNA can be sent to customers for their own use.