DNA extraction
Over the years, our method for DNA extraction has been optimised to ensure high quality and to produce a large amount of DNA. The samples can either be seeds or leaf material. The DNA can either be analysed at ScanBi Diagnostics or sent to the customer for their own use. Upon request, remaining DNA can always be sent to the customer within one (1) year of receipt of the samples.
Seeds
Two sub-samples of approx. 2 g each are used for the DNA extraction.
The two sub-samples are extracted separately. The homogenisation control is extracted in parallel with the samples.
Leaves
Pooled leaves: Up to 100 pooled leaves (pieces of 6-8 mm in diameter) are homogenised and followed by two (2) extractions of DNA from each pool.
Single leaves: Each leaflet (6-8 mm in diameter) is separately extracted for DNA. A single DNA extraction is performed per leaflet.
Upon request, 96-well plates for leaf sampling can be provided. Please contact ScanBi Diagnostics to get instructions how to sample and arrange logistics.
You can either order directly HERE or contact us by MAIL or PHONE (+46 40 69 28 001)